sgrna vector Search Results


puc57 sgrna expression vector  (Addgene inc)
95
Addgene inc puc57 sgrna expression vector
Puc57 Sgrna Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/10__1161_slash_hypertensionaha__116__08500-16-17-20?v=Addgene+inc
Average 95 stars, based on 1 article reviews
puc57 sgrna expression vector - by Bioz Stars, 2026-08
95/100 stars
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cas9 sgrna mediated knockout  (Addgene inc)
97
Addgene inc cas9 sgrna mediated knockout
Cas9 Sgrna Mediated Knockout, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/pm36861495-90-4-30?v=Addgene+inc
Average 97 stars, based on 1 article reviews
cas9 sgrna mediated knockout - by Bioz Stars, 2026-08
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abe7 10 max vrqr c  (Addgene inc)
91
Addgene inc abe7 10 max vrqr c
Abe7 10 Max Vrqr C, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/pmc10088465-458-25-30?v=Addgene+inc
Average 91 stars, based on 1 article reviews
abe7 10 max vrqr c - by Bioz Stars, 2026-08
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plasmids puc57 sa sgrna  (Addgene inc)
92
Addgene inc plasmids puc57 sa sgrna
Plasmids Puc57 Sa Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/pmc06002399-195-0-16?v=Addgene+inc
Average 92 stars, based on 1 article reviews
plasmids puc57 sa sgrna - by Bioz Stars, 2026-08
92/100 stars
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caggs ascpf1 2a gfp u6 sgrna cloning vector  (Addgene inc)
93
Addgene inc caggs ascpf1 2a gfp u6 sgrna cloning vector

Caggs Ascpf1 2a Gfp U6 Sgrna Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/pmc07659530-129-0-6?v=Addgene+inc
Average 93 stars, based on 1 article reviews
caggs ascpf1 2a gfp u6 sgrna cloning vector - by Bioz Stars, 2026-08
93/100 stars
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sgrna vector  (Addgene inc)
92
Addgene inc sgrna vector

Sgrna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/pmc10698695-207-2-13?v=Addgene+inc
Average 92 stars, based on 1 article reviews
sgrna vector - by Bioz Stars, 2026-08
92/100 stars
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vectors for sgrna expression  (BestGene Inc)
90
BestGene Inc vectors for sgrna expression

Vectors For Sgrna Expression, supplied by BestGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/pm30601807-362-2-14?v=BestGene+Inc
Average 90 stars, based on 1 article reviews
vectors for sgrna expression - by Bioz Stars, 2026-08
90/100 stars
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sgrna expression vector  (GenScript corporation)
90
GenScript corporation sgrna expression vector

Sgrna Expression Vector, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/pm32738650-55-38-59?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
sgrna expression vector - by Bioz Stars, 2026-08
90/100 stars
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sgrna vectors containing oryza sativa u6 promoter  (GenScript corporation)
90
GenScript corporation sgrna vectors containing oryza sativa u6 promoter
Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at <t>sgRNA</t> target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed <t>under</t> <t>Oryza</t> sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.
Sgrna Vectors Containing Oryza Sativa U6 Promoter, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/pmc08525377-75-0-16?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
sgrna vectors containing oryza sativa u6 promoter - by Bioz Stars, 2026-08
90/100 stars
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sgrna raav9 vectors  (Audentes Therapeutics)
90
Audentes Therapeutics sgrna raav9 vectors
Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at <t>sgRNA</t> target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed <t>under</t> <t>Oryza</t> sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.
Sgrna Raav9 Vectors, supplied by Audentes Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/pm36634841-157-37-1?v=Audentes+Therapeutics
Average 90 stars, based on 1 article reviews
sgrna raav9 vectors - by Bioz Stars, 2026-08
90/100 stars
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lentiviral sgrna expression vectors pxpr_brd043  (Broad Institute Inc)
90
Broad Institute Inc lentiviral sgrna expression vectors pxpr_brd043
Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at <t>sgRNA</t> target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed <t>under</t> <t>Oryza</t> sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.
Lentiviral Sgrna Expression Vectors Pxpr Brd043, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/pm35405016-439-27-42?v=Broad+Institute+Inc
Average 90 stars, based on 1 article reviews
lentiviral sgrna expression vectors pxpr_brd043 - by Bioz Stars, 2026-08
90/100 stars
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dual sgrna vector  (BestGene Inc)
90
BestGene Inc dual sgrna vector
Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at <t>sgRNA</t> target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed <t>under</t> <t>Oryza</t> sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.
Dual Sgrna Vector, supplied by BestGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+vector/bio_rxiv__2021__08__13__455988-114-4-17?v=BestGene+Inc
Average 90 stars, based on 1 article reviews
dual sgrna vector - by Bioz Stars, 2026-08
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Image Search Results


Journal: Cell Reports Medicine

Article Title: Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction

doi: 10.1016/j.xcrm.2020.100097

Figure Lengend Snippet:

Article Snippet: CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector , This study , Addgene 159281.

Techniques: Recombinant, Cytotoxicity Assay, Purification, Luminex, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Labeling, Plasmid Preparation, Software

Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at sgRNA target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed under Oryza sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.

Journal: Frontiers in Genome Editing

Article Title: Multiallelic, Targeted Mutagenesis of Magnesium Chelatase With CRISPR/Cas9 Provides a Rapidly Scorable Phenotype in Highly Polyploid Sugarcane

doi: 10.3389/fgeed.2021.654996

Figure Lengend Snippet: Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at sgRNA target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed under Oryza sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.

Article Snippet: sgRNA vectors containing Oryza sativa U6 promoter were designed and custom synthesized in the pUC57 backbone (Genscript, NJ, USA) to generate pUCMg12.

Techniques: Mutagenesis, In Vitro, Activity Assay, Cleavage Assay, Amplification, Plasmid Preparation, Control, Virus